Transformation and fusion of Streptococcus faecalis protoplasts
نویسندگان
چکیده
منابع مشابه
Formation of protoplasts from Streptococcus faecalis by lysozyme.
Bibb, William R. (University of North Carolina, Chapel Hill) and W. R. Straughn. Formation of protoplasts from Streptococcus faecalis by lysozyme. J. Bacteriol. 84:1094-1098. 1962.-Incubation of whole cells of Streptococcus faecalis F24 in the presence of the crystalline egg-white lysozyme and appropriate sucrose concentration resulted in the formation of discrete spherical structures. On dilut...
متن کاملTransformation of Streptococcus lactis Protoplasts by Plasmid DNA.
Polyethylene glycol-treated protoplasts prepared from Streptococcus lactis LM3302, a lactose-negative (Lac) derivative of S. lactis ML3, were transformed to lactose-fermenting ability by a transductionally shortened plasmid (pLM2103) coding for lactose utilization.
متن کاملHigh-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA.
Streptococcus lactis IL1403 protoplasts were transformed by plasmid pIL204 (5.5 kilobases), which conferred erythromycin resistance with an average efficiency of 5 X 10(6) transformants per microgram of supercoiled DNA. The procedure used and transformation efficiencies obtained were close to those described for Bacillus subtilis (G. Chang and S. N. Cohen, Mol. Gen. Genet. 168:111-115, 1979).
متن کاملcompactifications and representations of transformation semigroups
this thesis deals essentially (but not from all aspects) with the extension of the notion of semigroup compactification and the construction of a general theory of semitopological nonaffine (affine) transformation semigroup compactifications. it determines those compactification which are universal with respect to some algebric or topological properties. as an application of the theory, it is i...
15 صفحه اولaureus, and Streptococcus faecalis
SYNOPSIS The incidence of trimethoprim resistance in coliforms and multiresistant strains of Staphylococcus aureus isolated in Bristol from 1970 to 1972 is low-2-3 and 1 0% respectively. The resistance is probably intrinsic; there is no evidence that it is R-factor or plasmid mediated. A single mechanism that confers resistance to both trimethoprim and sulphamethoxazole has not been detected. N...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1985
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.162.1.92-97.1985